Electrochemical MIP sensor for butyrylcholinesterase

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dc.contributor.authorÖzçelikay, Göksu
dc.contributor.authorKurbanoğlu, Sevinç
dc.contributor.authorZhang, Xiaorong
dc.contributor.authorSöz, Çağla
dc.contributor.authorWollenberger, Ulla
dc.contributor.authorÖzkan, Sibel Ayşıl
dc.contributor.authorScheller, Frieder W.
dc.date.accessioned2021-01-08T21:51:21Z
dc.date.available2021-01-08T21:51:21Z
dc.date.issued2019
dc.departmentTAÜ, Fen Fakültesi, Malzeme Bilimi ve Teknolojileri Bölümüen_US
dc.descriptionSoz, Cagla/0000-0003-3942-070X; Wollenberger, Ulla/0000-0002-7088-8928en_US
dc.descriptionWOS:000507624500063en_US
dc.descriptionPubMed: 31801184en_US
dc.description.abstractMolecularly imprinted polymers (MIPs) mimic the binding sites of antibodies by substituting the amino acid-scaffold of proteins by synthetic polymers. In this work, the first MIP for the recognition of the diagnostically relevant enzyme butyrylcholinesterase (BuChE) is presented. The MIP was prepared using electropolymerization of the functional monomer o-phenylenediamine and was deposited as a thin film on a glassy carbon electrode by oxidative potentiodynamic polymerization. Rebinding and removal of the template were detected by cyclic voltammetry using ferricyanide as a redox marker. Furthermore, the enzymatic activity of BuChE rebound to the MIP was measured via the anodic oxidation of thiocholine, the reaction product of butyrylthiocholine. The response was linear between 50 pM and 2 nM concentrations of BuChE with a detection limit of 14.7 pM. In addition to the high sensitivity for BuChE, the sensor responded towards pseudo-irreversible inhibitors in the lower mM range.
dc.description.sponsorshipERA-Chemistry (2014) [61133]; Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany's Excellence StrategyGerman Research Foundation (DFG) [EXC 2008/1, 390540038]; Ankara University Erasmus Program; Ankara University UBED ProgramAnkara University
dc.description.sponsorshipThis work was supported by ERA-Chemistry (2014, 61133) and partially funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany's Excellence Strategy - EXC 2008/1 (UniSysCat) - 390540038 [Gefordert durch die Deutsche Forschungsgemeinschaft (DFG) im Rahmen der Exzellenzstrategie des Bundes und der Lander - EXC 2008/1 (UniSysCat) -390540038]. G.O., S.K. and S.A.O. gratefully acknowledge financial support from Ankara University Erasmus and UBED Programs.
dc.identifier.doi10.3390/polym11121970
dc.identifier.issn2073-4360
dc.identifier.issue12en_US
dc.identifier.scopus2-s2.0-85079150433
dc.identifier.scopusqualityQ1
dc.identifier.urihttp://doi.org/10.3390/polym11121970
dc.identifier.urihttps://hdl.handle.net/20.500.12846/175
dc.identifier.volume11en_US
dc.identifier.wosWOS:000507624500063
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorSöz, Çağla
dc.language.isoen
dc.publisherMdpi
dc.relation.ispartofPolymers
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectMolecularly Imprinted Polymersen_US
dc.subjectBiomimetic Sensorsen_US
dc.subjectButyrylcholinesteraseen_US
dc.subjectO-Phenylenediamineen_US
dc.subjectRivastigmineen_US
dc.titleElectrochemical MIP sensor for butyrylcholinesterase
dc.typeArticle

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