Electrochemical MIP sensor for butyrylcholinesterase
Yükleniyor...
Dosyalar
Tarih
2019
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Mdpi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Molecularly imprinted polymers (MIPs) mimic the binding sites of antibodies by substituting the amino acid-scaffold of proteins by synthetic polymers. In this work, the first MIP for the recognition of the diagnostically relevant enzyme butyrylcholinesterase (BuChE) is presented. The MIP was prepared using electropolymerization of the functional monomer o-phenylenediamine and was deposited as a thin film on a glassy carbon electrode by oxidative potentiodynamic polymerization. Rebinding and removal of the template were detected by cyclic voltammetry using ferricyanide as a redox marker. Furthermore, the enzymatic activity of BuChE rebound to the MIP was measured via the anodic oxidation of thiocholine, the reaction product of butyrylthiocholine. The response was linear between 50 pM and 2 nM concentrations of BuChE with a detection limit of 14.7 pM. In addition to the high sensitivity for BuChE, the sensor responded towards pseudo-irreversible inhibitors in the lower mM range.
Açıklama
Soz, Cagla/0000-0003-3942-070X; Wollenberger, Ulla/0000-0002-7088-8928
WOS:000507624500063
PubMed: 31801184
WOS:000507624500063
PubMed: 31801184
Anahtar Kelimeler
Molecularly Imprinted Polymers, Biomimetic Sensors, Butyrylcholinesterase, O-Phenylenediamine, Rivastigmine
Kaynak
Polymers
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
11
Sayı
12
