Enzymes as tools in MIP-Sensors
Yükleniyor...
Dosyalar
Tarih
2017
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Mdpi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Molecularly imprinted polymers (MIPs) have the potential to complement antibodies in bioanalysis, are more stable under harsh conditions, and are potentially cheaper to produce. However, the affinity and especially the selectivity of MIPs are in general lower than those of their biological pendants. Enzymes are useful tools for the preparation of MIPs for both low and high-molecular weight targets: As a green alternative to the well-established methods of chemical polymerization, enzyme-initiated polymerization has been introduced and the removal of protein templates by proteases has been successfully applied. Furthermore, MIPs have been coupled with enzymes in order to enhance the analytical performance of biomimetic sensors: Enzymes have been used in MIP-sensors as tracers for the generation and amplification of the measuring signal. In addition, enzymatic pretreatment of an analyte can extend the analyte spectrum and eliminate interferences.
Açıklama
Wollenberger, Ulla/0000-0002-7088-8928
WOS:000404532700001
WOS:000404532700001
Anahtar Kelimeler
Enzymatic Mip Synthesis, Template Digestion, Enzyme Tracer, Enzymatic Analyte Conversion, Molecularly Imprinted Polymers
Kaynak
Chemosensors
WoS Q Değeri
N/A
Scopus Q Değeri
Q2
Cilt
5
Sayı
2
