A Strep-Tag Imprinted Polymer Platform for Heterogenous Bio(electro)catalysis

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dc.authorid0000-0002-1465-9848
dc.contributor.authorYarman, Aysu
dc.contributor.authorWaffo, Armel F. T.
dc.contributor.authorKatz, Sagie
dc.contributor.authorBernitzky, Cornelius
dc.contributor.authorKovacs, Norbert
dc.contributor.authorBorrero, Paloma
dc.contributor.authorFrielingsdorf, Stefan
dc.contributor.authorSupala, Eszter
dc.contributor.authorDragelj, Jovan
dc.contributor.authorKurbanoğlu, Sevinç
dc.date.accessioned2024-12-27T17:33:01Z
dc.date.available2024-12-27T17:33:01Z
dc.date.issued2024
dc.departmentTAÜ, Fen Fakültesi, Moleküler Biyoteknoloji Bölümüen_US
dc.description.abstractMolecularly imprinted polymers (MIPs) are artificial receptors equipped with selective recognition sites for target molecules. One of the most promising strategies for protein MIPs relies on the exploitation of short surface-exposed protein fragments, termed epitopes, as templates to imprint binding sites in a polymer scaffold for a desired protein. However, the lack of highresolution structural data of flexible surface-exposed regions challenges the selection of suitable epitopes. Here, we addressed this drawback by developing a polyscopoletin-based MIP that recognizes recombinant proteins via imprinting of the widely used Strep-tag II affinity peptide (Strep-MIP). Electrochemistry, surfacesensitive IR spectroscopy, and molecular dynamics simulations were employed to ensure an utmost control of the Strep-MIP electrosynthesis. The functionality of this novel platform was verified with two Strep-tagged enzymes: an O2-tolerant [NiFe]-hydrogenase, and an alkaline phosphatase. The enzymes preserved their biocatalytic activities after multiple utilization confirming the efficiency of Strep-MIP as a general biocompatible platform to confine recombinant proteins for exploitation in biotechnology.
dc.identifier.citationYarman, A., Waffo, Armel F. T., Katz, S., Bernitzky, C., Kovacs, N., Borrero, P., Frielingsdorf, S., Supala, E., Dragelj, J., Kurbanoğlu, S. (2024). A Strep-Tag Imprinted Polymer Platform for Heterogenous Bio(electro)catalysis. Molecularly Imprinted Polymers, 63.
dc.identifier.doi10.1002/anie.202408979
dc.identifier.issn1433-7851
dc.identifier.issue63en_US
dc.identifier.scopus2-s2.0-85206915264
dc.identifier.urihttps://onlinelibrary.wiley.com/doi/10.1002/anie.202408979
dc.identifier.urihttps://hdl.handle.net/20.500.12846/1506
dc.identifier.wosWOS:001337572500001
dc.indekslendigikaynakWeb of Science
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofMolecularly Imprinted Polymers
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleA Strep-Tag Imprinted Polymer Platform for Heterogenous Bio(electro)catalysis
dc.typeArticle

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