A Strep-Tag Imprinted Polymer Platform for Heterogenous Bio(electro)catalysis
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Tarih
2024
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wiley
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Molecularly imprinted polymers (MIPs) are artificial receptors equipped with selective recognition sites for target molecules. One of the most promising strategies for protein MIPs relies on the exploitation of short surface-exposed protein fragments, termed epitopes, as templates to imprint binding sites in a polymer scaffold for a desired protein. However, the lack of highresolution structural data of flexible surface-exposed regions challenges the selection of suitable epitopes. Here, we addressed this drawback by developing a polyscopoletin-based MIP that recognizes recombinant proteins via imprinting of the widely used Strep-tag II affinity peptide (Strep-MIP). Electrochemistry, surfacesensitive IR spectroscopy, and molecular dynamics simulations were employed to ensure an utmost control of the Strep-MIP electrosynthesis. The functionality of this novel platform was verified with two Strep-tagged enzymes: an O2-tolerant [NiFe]-hydrogenase, and an alkaline phosphatase. The enzymes preserved their biocatalytic activities after multiple utilization confirming the efficiency of Strep-MIP as a general biocompatible platform to confine recombinant proteins for exploitation in biotechnology.
Açıklama
Anahtar Kelimeler
Kaynak
Molecularly Imprinted Polymers
WoS Q Değeri
Scopus Q Değeri
Cilt
Sayı
63
Künye
Yarman, A., Waffo, Armel F. T., Katz, S., Bernitzky, C., Kovacs, N., Borrero, P., Frielingsdorf, S., Supala, E., Dragelj, J., Kurbanoğlu, S. (2024). A Strep-Tag Imprinted Polymer Platform for Heterogenous Bio(electro)catalysis. Molecularly Imprinted Polymers, 63.
