Electrosynthesized molecularly imprinted polymer for laccase using the inactivated enzyme as the target

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dc.contributor.authorYarman, Aysu
dc.date.accessioned2021-01-08T21:51:24Z
dc.date.available2021-01-08T21:51:24Z
dc.date.issued2018
dc.departmentTAÜ, Fen Fakültesi, Moleküler Biyoteknoloji Bölümüen_US
dc.descriptionWOS:000428991300013en_US
dc.description.abstractThe first molecularly imprinted polymer (MIP) for the recognition of the copper-enzyme laccase was successfully prepared by electropolymerizing scopoletin in the presence of alkaline-inactivated enzyme. Laccase-MIP and the control polymer without laccase (nonimprinted polymer, NIP) were characterized by voltammetry using the redox marker ferricyanide. After electropolymerization, the signals for ferricyanide for both the MIP and the NIP were almost completely suppressed and increased after removal of the target from the polymer layer. Rebinding of both inactivated and active laccase decreased the ferricyanide peak currents to almost equal extent. The relative decrease of signal suppression approached saturation above 10 nM. Furthermore, the surface activity of rebound laccase toward the oxidation of catechol was investigated. The surface activity approached saturation above 10 nM, a value close to the value of the measurements with ferricyanide. Interaction of NIP with laccase brought about a six times smaller signal of catechol oxidation.
dc.identifier.doi10.1002/bkcs.11413
dc.identifier.endpage488en_US
dc.identifier.issn1229-5949
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-85042384957
dc.identifier.scopusqualityQ3
dc.identifier.startpage483en_US
dc.identifier.urihttp://doi.org/10.1002/bkcs.11413
dc.identifier.urihttps://hdl.handle.net/20.500.12846/226
dc.identifier.volume39en_US
dc.identifier.wosWOS:000428991300013
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorYarman, Aysu
dc.language.isoen
dc.publisherWiley-V C H Verlag Gmbh
dc.relation.ispartofBulletin Of The Korean Chemical Society
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectMolecularly Imprinted Polymersen_US
dc.subjectBiomimetic Sensorsen_US
dc.subjectLaccaseen_US
dc.subjectElectropolymerizationen_US
dc.subjectScopoletinen_US
dc.titleElectrosynthesized molecularly imprinted polymer for laccase using the inactivated enzyme as the target
dc.typeArticle

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