Development of a molecularly imprinted polymer-based electrochemical sensor for tyrosinase
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Dosyalar
Tarih
2018
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Scientific & Technical Research Council Turkey-TUBITAK
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
For the first time a molecularly imprinted polymer (MIP)-based sensor for tyrosinase is described. This sensor is based on the electropolymerization of scopoletin or o-phenylenediamine in the presence of tyrosinase from mushrooms, which has a high homology to the human enzyme. The template was removed either by treatment with proteinase K or by alkaline treatment. The measuring signal was generated either by measuring the formation of a product by the target enzyme or by evaluation of the permeability of the redox marker ferricyanide. The o-phenylenediamine-based MIP sensor has a linear measuring range up to 50 nM of tyrosinase with a limit of detection of 3.97 nM (R-2 = 0.994) and shows good discrimination towards other proteins, e.g., bovine serum albumin and cytochrome c.
Açıklama
WOS:000431245300012
Anahtar Kelimeler
Molecularly Imprinted Polymers, Biomimetic Sensors, Tyrosinase, Electropolymerization, Scopoletin, Ophenylenediamine
Kaynak
Turkish Journal Of Chemistry
WoS Q Değeri
Q3
Scopus Q Değeri
Q3
Cilt
42
Sayı
2
