Effect of various carbon electrodes on MIP-Based sensing proteins using poly(Scopoletin): A case study of ferritin

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dc.contributor.authorYarman, Ayşe
dc.date.accessioned2024-10-04T17:36:03Z
dc.date.available2024-10-04T17:36:03Z
dc.date.issued2024
dc.departmentTAÜ, Fen Fakültesi, Moleküler Biyoteknoloji Bölümüen_US
dc.description.abstractSensitivity in the sub-nanomolar concentration region is required to determine important protein biomarkers, e.g., ferritin. As a prerequisite for high sensitivity, in this paper, the affinity of the functional monomer to the macromolecular target ferritin in solution was compared with the value for the respective molecularly imprinted polymer (MIP)-based electrodes, and the influence of various surface modifications of the electrode was investigated. The analytical performance of ferritin sensing was investigated using three different carbon electrodes (screen-printed carbon electrodes, singlewalled-carbon-nanotube-modified screen-printed carbon electrodes, and glassy carbon electrodes) covered with a scopoletin-based MIP layer. Regardless of the electrode type, the template molecule ferritin was mixed with the functional monomer scopoletin, and electropolymerization was conducted using multistep amperometry. All stages of MIP preparation were followed by evaluating the diffusional permeability of the redox marker ferricyanide/ferrocyanide through the polymer layer by differential pulse voltammetry. The best results were obtained with glassy carbon electrodes. The MIP sensor responded up to 0.5 µM linearly with a Kd of 0.30 µM. Similar results were also obtained in solution upon the interaction of scopoletin and ferritin using fluorescence spectroscopy, resulting in the quenching of the scopoletin signal, with a calculated Kd of 0.81 µM. Moreover, the binding of 1 µM ferritin led to 49.6% suppression, whereas human serum albumin caused 8.6% suppression.
dc.identifier.citationYarman, A. (2024). Effect of various carbon electrodes on MIP-Based sensing proteins using poly(Scopoletin): A case study of ferritin. Biomimetics, 9 (426). 1-11.
dc.identifier.doi10.3390/biomimetics9070426
dc.identifier.endpage11en_US
dc.identifier.issn2313-7673
dc.identifier.issue426en_US
dc.identifier.scopus2-s2.0-85199659377
dc.identifier.startpage1en_US
dc.identifier.urihttps://www.mdpi.com/2313-7673/9/7/426
dc.identifier.urihttps://hdl.handle.net/20.500.12846/1384
dc.identifier.volume9en_US
dc.identifier.wosWOS:001276719700001
dc.indekslendigikaynakScopus
dc.language.isoen
dc.relation.ispartofBiomimetics
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectFerritinen_US
dc.subjectMolecularly imprinted polymersen_US
dc.subjectBiomimetic sensorsen_US
dc.subjectScreen-printed electrodesen_US
dc.subjectGlassy carbon electrodesen_US
dc.titleEffect of various carbon electrodes on MIP-Based sensing proteins using poly(Scopoletin): A case study of ferritin
dc.typeArticle

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