“Out of pocket” protein binding—A dilemma of epitope imprinted polymers revealed for human hemoglobin

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dc.authorid0000-0002-1465-9848
dc.contributor.authorZhang, Xiaorong
dc.contributor.authorCaserta, Giorgio
dc.contributor.authorSupala, Eszter
dc.contributor.authorWollenberger, Ulla
dc.contributor.authorGyurcsányi, Róbert E.
dc.contributor.authorZebger, Ingo
dc.contributor.authorScheller, Frieder W.
dc.contributor.authorYarman, Aysu
dc.date.accessioned2024-04-24T18:20:32Z
dc.date.available2024-04-24T18:20:32Z
dc.date.issued2021
dc.departmentTAÜ, Fen Fakültesi, Moleküler Biyoteknoloji Bölümüen_US
dc.description.abstractThe epitope imprinting approach applies exposed peptides as templates to synthesize Molecularly Imprinted Polymers (MIPs) for the recognition of the parent protein. While generally the template protein binding to such MIPs is considered to occur via the epitope-shaped cavities, unspecific interactions of the analyte with non-imprinted polymer as well as the detection method used may add to the complexity and interpretation of the target rebinding. To get new insights on the effects governing the rebinding of analytes, we electrosynthesized two epitope-imprinted polymers using the N-terminal pentapeptide VHLTP-amide of human hemoglobin (HbA) as the template. MIPs were prepared either by single-step electrosynthesis of scopoletin/pentapeptide mixtures or electropolymerization was performed after chemisorption of the cysteine extended VHLTP peptide. Rebinding of the target peptide and the parent HbA protein to the MIP nanofilms was quantified by square wave voltammetry using a redox probe gating, surface enhanced infrared absorption spectroscopy, and atomic force microscopy. While binding of the pentapeptide shows large influence of the amino acid sequence, all three methods revealed strong non-specific binding of HbA to both polyscopoletin-based MIPs with even higher affinities than the target peptides.
dc.identifier.citationZhang, X., Caserta, G., Supala, E., Wollenberger, U., Gyurcsányi, Róbert E., Zebger, I., Scheller, Frieder W., Yarman, A. (2021). “Out of pocket” protein binding—A dilemma of epitope imprinted polymers revealed for human hemoglobin. Chemosensors, 9 (6).
dc.identifier.doi10.3390/chemosensors9060128
dc.identifier.issue6en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12846/1150
dc.identifier.volume9en_US
dc.language.isoen
dc.relation.ispartofChemosensors
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectMolecularly imprinted polymersen_US
dc.subjectEpitope imprintingen_US
dc.subjectNon-specific bindingen_US
dc.subjectRedox gatingen_US
dc.subjectSEIRA spectroelectrochemistryen_US
dc.title“Out of pocket” protein binding—A dilemma of epitope imprinted polymers revealed for human hemoglobin
dc.typeArticle

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