Insights in electrosynthesis, target binding, and stability of peptide-imprinted polymer nanofilms

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dc.authorid0000-0002-1465-9848
dc.contributor.authorYarman, Aysu
dc.contributor.authorCaserta, Giorgio
dc.contributor.authorGyurcsányi, Róbert E.
dc.contributor.authorWollenberger, Ulla
dc.contributor.authorZhang, Xiaorong
dc.contributor.authorZebger, Ingo
dc.contributor.authorSupala, Eszter
dc.contributor.authorScheller, Frieder W.
dc.date.accessioned2024-04-25T12:11:22Z
dc.date.available2024-04-25T12:11:22Z
dc.date.issued2021
dc.departmentTAÜ, Fen Fakültesi, Moleküler Biyoteknoloji Bölümüen_US
dc.description.abstractMolecularly imprinted polymer (MIP) nanofilms have been successfully implemented for the recognition of different target molecules: however, the underlying mechanistic details remained vague. This paper provides new insights in the preparation and binding mechanism of electrosynthesized peptide-imprinted polymer nanofilms for selective recognition of the terminal pentapeptides of the ß-chains of human adult hemoglobin, HbA, and its glycated form HbA1c. To differentiate between peptides differing solely in a glucose adduct MIP nanofilms were prepared by a two-step hierarchical electrosynthesis that involves first the chemisorption of a cysteinyl derivative of the pentapeptide followed by electropolymerization of scopoletin. This approach was compared with a random single-step electrosynthesis using scopoletin/pentapeptide mixtures. Electrochemical monitoring of the peptide binding to the MIP nanofilms by means of redox probe gating revealed a superior affinity of the hierarchical approach with a Kd value of 64.6 nM towards the related target. Changes in the electrosynthesized non-imprinted polymer and MIP nanofilms during chemical, electrochemical template removal and rebinding were substantiated in situ by monitoring the characteristic bands of both target peptides and polymer with surface enhanced infrared absorption spectroscopy. This rational approach led to MIPs with excellent selectivity and provided key mechanistic insights with respect to electrosynthesis, rebinding and stability of the formed MIPs
dc.identifier.citationYarman, A., Caserta, G., Gyurcsányi, Róbert E., Wollenberger, U., Zhang, X., Zebger, I., Supala, E., Scheller, Frieder W. (2021). Insights in electrosynthesis, target binding, and stability of peptide-imprinted polymer nanofilms. Electrochimica Acta, 381.
dc.identifier.doi10.1016/j.electacta.2021.138236
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0013468621005260?via%3Dihub
dc.identifier.urihttps://hdl.handle.net/20.500.12846/1151
dc.identifier.volume381en_US
dc.language.isoen
dc.relation.ispartofElectrochimica Acta
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectSEIRA spectroelectrochemistryen_US
dc.subjectPeptide imprintingen_US
dc.subjectElectrosynthesisen_US
dc.subjectMIPen_US
dc.subjectGlycated peptideen_US
dc.titleInsights in electrosynthesis, target binding, and stability of peptide-imprinted polymer nanofilms
dc.typeArticle

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