How reliable is the electrochemical readout of MIP sensors?
Yükleniyor...
Dosyalar
Tarih
2020
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Sensors,
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Electrochemical methods offer the simple characterization of the synthesis of molecularly imprinted polymers (MIPs) and the readouts of target binding. The binding of electroinactive analytes can be detected indirectly by their modulating effect on the diffusional permeability of a redox marker through thin MIP films. However, this process generates an overall signal, which may include nonspecific interactions with the nonimprinted surface and adsorption at the electrode surface in addition to (specific) binding to the cavities. Redox-active low-molecular-weight targets and metalloproteins enable a more specific direct quantification of their binding to MIPs by measuring the faradaic current. The in situ characterization of enzymes, MIP-based mimics of redox enzymes or enzyme-labeled targets, is based on the indication of an electroactive product. This approach allows the determination of both the activity of the bio(mimetic) catalyst and of the substrate concentration.
Açıklama
Anahtar Kelimeler
Molecularly imprinted polymers, Electropolymerization, Direct electron transfer, Catalysis, Redox marker, Gate effect
Kaynak
WoS Q DeÄŸeri
Scopus Q DeÄŸeri
Cilt
20
Sayı
9
Künye
Yarman A, Scheller F. W. (2020). How reliable is the electrochemical readout of MIP sensors?. Sensors, 20(9), 2677.
