Yazar "İş, Şeyma" seçeneğine göre listele

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  • Küçük Resim
    Öğe
    Çörek Otu’nun (Nigella sativa) biyolojik ve farmakolojik özellikleri
    (Mersin Üniversitesi Tıp Fakültesi Lokman Hekim Tıp Tarihi ve Folklorik Tıp Dergisi, 2023) İş, Şeyma; Beyatlı, Ahmet
    Ranunculaceae familyasının bir üyesi olan Nigella sativa, Güney Avrupa ve Batı Asya’da doğal olarak yetişen ve dünyanın birçok bölgesinde kültive edilen tek yıllık bir bitkidir. Dünya mutfaklarında baharat olarak kullanılmasının yanı sıra binlerce yıldır Unani, Ayurveda, Siddha ve Tıbb-ı Nebevî gibi Geleneksel Tıp Sistemleri’nde kullanılan ve kutsallık atfedilen bu şifâlı bitki günümüzde de gastrointestinal rahatsızlıklar, cilt hastalıkları, diyabet ve kanser hastalıklarında ve ayrıca kozmetik amaçlar ile saç dökülmesine karşı ve yaşlanma karşıtı cilt bakımında kullanılmaktadır. Yaygın kullanımına rağmen, tıbbî amaçlı kullanılan Çörek Otu (Nigella sativa) bitkisi sıklıkla belirgin bir özellik olarak dikenimsi çanak yaprakları olan ve süs bitkisi olarak kullanılan Şam Çörek Otu (Nigella damascena) bitkisi ile karıştırılmaktadır; ki bu karışıklık maalesef bilimsel literatürde de söz konusudur. Bu çalışma ile Nigella türlerinin doğru tanınması ve tanımlanması amacıyla bir farkındalık oluşturmak, Nigella sativa’nın içerdiği timokinon, p-simen, karvakrol, timol ve trans-anetol gibi biyoaktif fitokimyasalların farmakolojik etkilerini özetlemek ve ayrıca son yıllarda yapılan araştırmaların değerlendirilmesi hedeflenmiştir.
  • Küçük Resim
    Öğe
    Identification of deleterious non-synonymous single nucleotide polymorphisms in the mRNA decay activator ZFP36L2
    (2024) Akçeşme, Betül; Hekimoğlu, Hilal; Chirasani, Venkat R.; İş, Şeyma; Atmaca, Habibe Nur; Waldern, Justin M.; Ramos, Silvia B. V.
    More than 4,000 single nucleotide polymorphisms (SNP) variants have been identified in the human ZFP36L2 gene, however only a few have been studied in the context of protein function. The tandem zinc finger domain of ZFP36L2, an RNA binding protein, is the functional domain that binds to its target mRNAs. This protein/RNA interaction triggers mRNA degradation, controlling gene expression. We identified 32 non-synonymous SNPs (nsSNPs) in the tandem zinc finger domain of ZFP36L2 that could have possible deleterious impacts in humans. Using different bioinformatic strategies, we prioritized five among these 32 nsSNPs, namely rs375096815, rs1183688047, rs1214015428, rs1215671792 and rs920398592 to be validated. When we experimentally tested the functionality of these protein variants using gel shift assays, all five (Y154H, R160W, R184C, G204D, and C206F) resulted in a dramatic reduction in RNA binding compared to the WT protein. To understand the mechanistic effect of these variants on the protein/RNA interaction, we employed DUET, DynaMut and PyMOL to investigate structural changes in the protein. Additionally, we conducted Molecular Docking and Molecular Dynamics Simulations to fine tune the active behaviour of this biomolecular system at an atomic level. Our results propose atomic explanations for the impact of each of these five genetic variants identified.
  • Küçük Resim
    Öğe
    Identification of nucleotide patterns in meconium microbiota to improve the management of meconium aspiration syndrome in clinical practice
    (Institute for Genetic Engineering and Biotechnology, University of Sarajevo, 2020) Akçeşme, Faruk Berat; İş, Şeyma
    For many years, airways, lungs and meconium, which is a thick green substance and accumulates in the baby’s intestines during pregnancy, have been assumed to be sterile. However, this assumption has been revised by the development of microbiota analysis, which is used for the identification of microorganisms as a new promising technique; it has been found that various microorganisms exist in the lungs and in the meconium fluid. Meconium Aspiration Syndrome (MAS) as a common neonatal problem can give rise to the development of lung infections in newborns and even cases leading to death are encountered. Various strategies regarding antibiotic therapy have been developed against the risk of infection, but positive outcome could not be obtained for each case. In this study, 16S rRNA gene sequences of bacteria existing in meconium microbiota and infected lung microbiota were subjected to comparative sequence analysis. Our aim was to identify positions of nucleotide patterns between the hypervariable regions of the bacterial 16S rRNA gene sequences that could provide similar functions among bacterial groups. Furthermore, similarity analysis was conducted to identify molecular signatures via phylogenetic framework to understand the etiology of the infections after MAS. Interestingly, Bifidobacteria which are used as probiotics were found to be similar to Actynomyces which are known as opportunistic pathogens. Furthermore, Clostridium leptum was associated with pulmonary inflammation for the first time. This study proposes the usage of microbiota analysis to improve the MAS management in clinical practice.