Yarman, AysuÖzçelikay, GöksuKurbanoğlu, SevinçScheller, Frieder W.Ozkan, Sibel A.2024-04-252024-04-252020Yarman, A., Özçelikay, G., Kurbanoğlu, S., Scheller, Frieder W., Ozkan, Sibel A. (2020). Au-Pt nanoparticles based molecularly imprinted nanosensor for electrochemical detection of the lipopeptide antibiotic drug Daptomycin, Sensors and Actuators B: Chemical, 320.https://hdl.handle.net/20.500.12846/1153In this work, a novel electrochemical molecularly imprinted polymer (MIP) sensor for the detection of the lipopeptide antibiotic Daptomycin (DAP) is presented which integrates gold decorated platinum nanoparticles (Au-Pt NPs) into the nanocomposite film. The sensor was prepared by electropolymerization of o-phenylenediamine (o-PD) in the presence of DAP using cyclic voltammetry. Cyclic voltammetry and differential pulse voltammetry were applied to follow the changes in the MIP-layer related to rebinding and removal of the target DAP by using the redox marker [Fe(CN)6] 3?/4– . Under optimized operational conditions, the MIP/Au-Pt NPs/ GCE nanosensor exhibits a linear response in the range of 1-20 pM towards DAP. The limit of detection and limit of quantification were determined to be 0.161pM ± 0.012 and 0.489pM ± 0.012, respectively. The sensitivity towards the antibiotics Vancomycin and Erythromycin and the amino acids glycine and tryptophan was below 7 percent as compared with DAP. Moreover, the nanosensor was also successfully used for the detection of DAP in deproteinated human serum samples.eninfo:eu-repo/semantics/openAccessMolecularly imprinted polymerDaptomycinPlatinum nanoparticlesGold nanoparticlesModified electrodesArticle32010.1016/j.snb.2020.128285