Yarman, Aysu2021-01-082021-01-0820181300-0527http://doi.org/10.3906/kim-1708-68https://hdl.handle.net/20.500.12846/235WOS:000431245300012For the first time a molecularly imprinted polymer (MIP)-based sensor for tyrosinase is described. This sensor is based on the electropolymerization of scopoletin or o-phenylenediamine in the presence of tyrosinase from mushrooms, which has a high homology to the human enzyme. The template was removed either by treatment with proteinase K or by alkaline treatment. The measuring signal was generated either by measuring the formation of a product by the target enzyme or by evaluation of the permeability of the redox marker ferricyanide. The o-phenylenediamine-based MIP sensor has a linear measuring range up to 50 nM of tyrosinase with a limit of detection of 3.97 nM (R-2 = 0.994) and shows good discrimination towards other proteins, e.g., bovine serum albumin and cytochrome c.eninfo:eu-repo/semantics/openAccessMolecularly Imprinted PolymersBiomimetic SensorsTyrosinaseElectropolymerizationScopoletinOphenylenediamineArticle42210.3906/kim-1708-68346354Q3Q3WOS:0004312453000122-s2.0-85044785995531145