Thomas, Pinar B.Kaluc, NurCavli, Irmak N.Tuna, Bilge G.2025-02-202025-02-2020250378-10971574-6968https://doi.org/10.1093/femsle/fnae109https://hdl.handle.net/20.500.12846/1614Chronological lifespan (CLS) in budding yeast Saccharomyces cerevisiae, which is defined as the time nondividing cells in saturation remain viable, has been utilized as a model to study post-mitotic aging in mammalian cells. CLS is closely related to entry into and maintenance of a quiescent state. Many rearrangements that direct the quiescent state enhance the ability of cells to endure several types of stress. Small ubiquitin-like modifier (SUMO)-targeted ubiquitin ligases (STUbLs) play a critical role in mediating an adaptive response to various stresses. In this study, we investigated the effect of a STUbL, Slx5/Slx8, on CLS in budding yeast. We showed that both SLX5 and SLX8 deletions accelerate chronological aging, resulting in a decreased maximum and mean lifespan. slx5 Delta cells were capable of entering or maintaining a quiescent state during aging. On the other hand, aging slx5 Delta and slx8 Delta cells had both increased spontaneous mutation accumulation. Our data together indicate that Slx5/Slx8 STUbL is required for normal rate of aging by preventing increased spontaneous mutation accumulation during aging. Slx5/Slx8 STUbL deficiency accelerates aging in yeast.eninfo:eu-repo/semantics/closedAccessSlx5/Slx8 STUbL (SUMO-targeted ubiquitin ligase)chronological agingquiescencemutation accumulationSaccharomyces cerevisiaeArticle37210.1093/femsle/fnae109Q3Q3WOS:0013933185000012-s2.0-8521514463239730145