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dc.contributor.authorYarman, Aysu
dc.contributor.authorJetzschmann, Katharina J.
dc.contributor.authorNeumann, Bettina
dc.contributor.authorZhang, Xiaorong
dc.contributor.authorWollenberger, Ulla
dc.contributor.authorCordin, Aude
dc.contributor.authorScheller, Frieder W.
dc.date.accessioned2021-01-08T21:51:25Z
dc.date.available2021-01-08T21:51:25Z
dc.date.issued2017
dc.identifier.issn2227-9040
dc.identifier.urihttp://doi.org/10.3390/chemosensors5020011
dc.identifier.urihttps://hdl.handle.net/20.500.12846/247
dc.descriptionWollenberger, Ulla/0000-0002-7088-8928en_US
dc.descriptionWOS:000404532700001en_US
dc.description.abstractMolecularly imprinted polymers (MIPs) have the potential to complement antibodies in bioanalysis, are more stable under harsh conditions, and are potentially cheaper to produce. However, the affinity and especially the selectivity of MIPs are in general lower than those of their biological pendants. Enzymes are useful tools for the preparation of MIPs for both low and high-molecular weight targets: As a green alternative to the well-established methods of chemical polymerization, enzyme-initiated polymerization has been introduced and the removal of protein templates by proteases has been successfully applied. Furthermore, MIPs have been coupled with enzymes in order to enhance the analytical performance of biomimetic sensors: Enzymes have been used in MIP-sensors as tracers for the generation and amplification of the measuring signal. In addition, enzymatic pretreatment of an analyte can extend the analyte spectrum and eliminate interferences.en_US
dc.description.sponsorshipDeutsche Forschungsgemeinschaft (DFG) within the framework of the German Excellence Initiative UnicatGerman Research Foundation (DFG) [EXC 314]; ERA-Chemistry [61133]; Turkish-German University Scientific Research Projects Commission [2016BF0011]; European Union FP7 Marie Curie programme (CHEBANA)European Union (EU) [PITN-GA-2010-264772]; Regional Council of Picardy (BIOMIP project)en_US
dc.description.sponsorshipThe authors gratefully acknowledge financial support from Deutsche Forschungsgemeinschaft (DFG) within the framework of the German Excellence Initiative Unicat (EXC 314), ERA-Chemistry (2014, 61133), and Turkish-German University Scientific Research Projects Commission under the Grant No. 2016BF0011 for financial support. KH and AFC acknowledge the European Union FP7 Marie Curie programme (CHEBANA, PITN-GA-2010-264772) and the Regional Council of Picardy (BIOMIP project) for funding.en_US
dc.language.isoengen_US
dc.publisherMdpien_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectEnzymatic Mip Synthesisen_US
dc.subjectTemplate Digestionen_US
dc.subjectEnzyme Traceren_US
dc.subjectEnzymatic Analyte Conversionen_US
dc.subjectMolecularly Imprinted Polymersen_US
dc.titleEnzymes as tools in MIP-Sensorsen_US
dc.typeReviewen_US
dc.relation.journalChemosensorsen_US
dc.identifier.volume5en_US
dc.identifier.issue2en_US
dc.relation.publicationcategoryotheren_US
dc.contributor.departmentTAÜ, Fen Fakültesi, Moleküler Biyoteknoloji Bölümüen_US
dc.contributor.institutionauthorYarman, Aysu
dc.identifier.doi10.3390/chemosensors5020011


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