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Development of a molecularly imprinted polymer-based electrochemical sensor for tyrosinase

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info:eu-repo/semantics/openAccess

Date

2018

Author

Yarman, Aysu

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Abstract

For the first time a molecularly imprinted polymer (MIP)-based sensor for tyrosinase is described. This sensor is based on the electropolymerization of scopoletin or o-phenylenediamine in the presence of tyrosinase from mushrooms, which has a high homology to the human enzyme. The template was removed either by treatment with proteinase K or by alkaline treatment. The measuring signal was generated either by measuring the formation of a product by the target enzyme or by evaluation of the permeability of the redox marker ferricyanide. The o-phenylenediamine-based MIP sensor has a linear measuring range up to 50 nM of tyrosinase with a limit of detection of 3.97 nM (R-2 = 0.994) and shows good discrimination towards other proteins, e.g., bovine serum albumin and cytochrome c.

Source

Turkish Journal Of Chemistry

Volume

42

Issue

2

URI

http://doi.org/10.3906/kim-1708-68
https://hdl.handle.net/20.500.12846/235

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  • Moleküler Biyoteknoloji [46]
  • Scopus İndeksli Yayınlar Koleksiyonu [278]
  • WoS İndeksli Yayınlar Koleksiyonu [315]



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