Moleküler Biyoteknolojihttps://hdl.handle.net/20.500.12846/4872024-03-29T09:45:34Z2024-03-29T09:45:34ZÇörek Otu’nun (Nigella sativa) biyolojik ve farmakolojik özellikleriİş, Şeymahttps://hdl.handle.net/20.500.12846/9272024-03-27T06:20:08Z2023-01-01T00:00:00ZÇörek Otu’nun (Nigella sativa) biyolojik ve farmakolojik özellikleri
İş, Şeyma
Ranunculaceae familyasının bir üyesi olan Nigella sativa, Güney Avrupa ve Batı Asya’da doğal olarak yetişen ve dünyanın birçok
bölgesinde kültive edilen tek yıllık bir bitkidir. Dünya mutfaklarında baharat olarak kullanılmasının yanı sıra binlerce yıldır Unani,
Ayurveda, Siddha ve Tıbb-ı Nebevî gibi Geleneksel Tıp Sistemleri’nde kullanılan ve kutsallık atfedilen bu şifâlı bitki günümüzde de
gastrointestinal rahatsızlıklar, cilt hastalıkları, diyabet ve kanser hastalıklarında ve ayrıca kozmetik amaçlar ile saç dökülmesine karşı
ve yaşlanma karşıtı cilt bakımında kullanılmaktadır. Yaygın kullanımına rağmen, tıbbî amaçlı kullanılan Çörek Otu (Nigella sativa) bitkisi
sıklıkla belirgin bir özellik olarak dikenimsi çanak yaprakları olan ve süs bitkisi olarak kullanılan Şam Çörek Otu (Nigella damascena)
bitkisi ile karıştırılmaktadır; ki bu karışıklık maalesef bilimsel literatürde de söz konusudur. Bu çalışma ile Nigella türlerinin doğru
tanınması ve tanımlanması amacıyla bir farkındalık oluşturmak, Nigella sativa’nın içerdiği timokinon, p-simen, karvakrol, timol ve
trans-anetol gibi biyoaktif fitokimyasalların farmakolojik etkilerini özetlemek ve ayrıca son yıllarda yapılan araştırmaların
değerlendirilmesi hedeflenmiştir.; Nigella sativa, a member of the Ranunculaceae family, is an annual herb that grows naturally in Southern Europe and Western Asia
and is cultivated in many parts of the world. In addition to being used as a spice in world cuisines, this medicinal plant, which has
been used for thousands of years in Traditional Medicine Systems such as Unani, Ayurveda, Siddha and prophetic medicine aṭ-Tibb
an-Nabawī and attributed to holiness, is still used in gastrointestinal disorders, skin diseases, diabetes and cancer diseases, and also
for cosmetic purposes such as anti-hair loss and anti-aging skin care. Despite its widespread use, the Black Cumin (Nigella sativa)
plant, which is used for medicinal purposes, is often confused with the Love-in-a-mist (Nigella damascena) plant, which has thorny
sepals as a distinctive feature and is used as an ornamental plant; unfortunately, this confusion also exists in the scientific literature.
With this study, it was aimed to raise awareness for the correct knowledge and identification of Nigella species, to summarize the
pharmacological effects of bioactive phytochemicals such as thymoquinone, p-cymene, carvacrol, thymol and trans-anethole
contained in Nigella sativa, and also to evaluate the studies conducted in recent years.
2023-01-01T00:00:00ZInducible overexpression of a FAM3C/ILEI transgene has pleiotropic effects with shortened life span, liver fibrosis and anemia in miceUluca, BetülSchmidt, UlrikeVokic, IvaMalik, BarizahKolbeI, ThomasLassnig, CarolineHolcmann, MartinMoreno-Viedma, VeronicaRobl, BernhardMuhlberge, CarinaGotthard, DagmarSibilia, MariaMüller, MathiasCsisza, AgnesRülick, Thomashttps://hdl.handle.net/20.500.12846/8872024-03-20T13:11:15Z2023-01-01T00:00:00ZInducible overexpression of a FAM3C/ILEI transgene has pleiotropic effects with shortened life span, liver fibrosis and anemia in mice
Uluca, Betül; Schmidt, Ulrike; Vokic, Iva; Malik, Barizah; KolbeI, Thomas; Lassnig, Caroline; Holcmann, Martin; Moreno-Viedma, Veronica; Robl, Bernhard; Muhlberge, Carina; Gotthard, Dagmar; Sibilia, Maria; Müller, Mathias; Csisza, Agnes; Rülick, Thomas
Klymkowsky, Michael
FAM3C/ILEI is an important factor in epithelial-to-mesenchymal transition (EMT) induction,
tumor progression and metastasis. Overexpressed in many cancers, elevated ILEI levels
and secretion correlate with poor patient survival. Although ILEI’s causative role in invasive
tumor growth and metastasis has been demonstrated in several cellular tumor models,
there are no available transgenic mice to study these effects in the context of a complex
organism. Here, we describe the generation and initial characterization of a Tet-ON inducible Fam3c/ILEI transgenic mouse strain. We find that ubiquitous induction of ILEI overexpression (R26-ILEIind) at weaning age leads to a shortened lifespan, reduced body weight
and microcytic hypochromic anemia. The anemia was reversible at a young age within a
week upon withdrawal of ILEI induction. Vav1-driven overexpression of the ILEIind transgene in all hematopoietic cells (Vav-ILEIind) did not render mice anemic or lower overall fitness, demonstrating that no intrinsic mechanisms of erythroid development were
dysregulated by ILEI and that hematopoietic ILEI hyperfunction did not contribute to death.
Reduced serum iron levels of R26-ILEIind mice were indicative for a malfunction in iron
uptake or homeostasis. Accordingly, the liver, the main organ of iron metabolism, was
severely affected in moribund ILEI overexpressing mice: increased alanine transaminase
and aspartate aminotransferase levels indicated liver dysfunction, the liver was reduced in
size, showed increased apoptosis, reduced cellular iron content, and had a fibrotic phenotype. These data indicate that high ILEI expression in the liver might reduce hepatoprotection and induce liver fibrosis, which leads to liver dysfunction, disturbed iron metabolism and
eventually to death. Overall, we show here that the novel Tet-ON inducible Fam3c/ILEI transgenic mouse strain allows tissue specific timely controlled overexpression of ILEI and
thus, will serve as a versatile tool to model the effect of elevated ILEI expression in diverse
tissue entities and disease conditions, including cancer.
2023-01-01T00:00:00ZMolekularer abdruck oder selektion bei der erzeugung biomimetischer specifyerOktay, AyselMenger, Marcus M.Yarman, AyşeScheller, Frieder W.https://hdl.handle.net/20.500.12846/8762024-03-20T07:57:29Z2023-01-01T00:00:00ZMolekularer abdruck oder selektion bei der erzeugung biomimetischer specifyer
Oktay, Aysel; Menger, Marcus M.; Yarman, Ayşe; Scheller, Frieder W.
Zum Ersatz oder auch zur Ergänzung von Antikörpern für niedermolekulare Substanzen wie Antibiotika, Umweltgifte und Pharmaka, aber auch für Proteinbiomarker, Viren und Mikroorganismen in Trennungstechniken, Diagnostik und Therapie, wurden Binder moleküle auf der Basis von Oligonukleotiden (Aptamere) mittels des SELEXVerfahrens und voll synthetische „Molekular Geprägte (Imprinted) Polymere“ (MIPs) entwickelt. Diese Specifyer können ohne Versuchstiere hergestellt werden und erreichen zu Antikörpern vergleichbare Affi nitäten. Sie konnten bereits in zahlreiche Applikationen transferiert werden, aber ihre Synthesekonzepte – Abformung vs. Selektion – können aufgrund ihrer jeweiligen Molekülbasis unterschiedlicher kaum sein.
2023-01-01T00:00:00Z3d printing personalized treatment methods for bone tissue engineering applicationsOktay, AyselOktay, BüşraBingöl, Ayşe BetülÜstündağ, Cem Bülenthttps://hdl.handle.net/20.500.12846/8752024-03-20T07:57:23Z2023-01-01T00:00:00Z3d printing personalized treatment methods for bone tissue engineering applications
Oktay, Aysel; Oktay, Büşra; Bingöl, Ayşe Betül; Üstündağ, Cem Bülent
Bone injuries and deformities are one of the major health problems worldwide. To overcome
this problem, bone tissue engineering focuses on producing synthetic bones that can be used to
treat patients with bone injuries or deformities. Personalized 3D printing is used to produce
customized bone prostheses based on the size and shape of the patient's bone damage. Printed
in accordance with software-generated algorithms, 3D printing enables the creation of patientspecific bone implants that precisely fit the individual's specific anatomy. This method, which
allows 3D-printed bone implants to be tailored to the specific needs of the patient, aims to
improve treatment outcomes and reduce the risk of complications associated with incompatible
standard implants. Biological materials such as biocompatible polymers and bioactive ceramics
can be used in the 3D printing process to ensure that the prosthesis is similar to the patient's
existing bone structure and to mimic the biological and mechanical properties of the tissue. In
addition, natural bone biopolymers, bioceramics, biological materials and cells are used
together. The inclusion of stem cells and growth factors for the production of biofunctional
implants can stimulate bone regeneration and accelerate the healing process. Bone tissue
engineering, which combines advanced 3D printing technologies with personalized treatment
methods, is an interesting application in the field of regenerative medicine. This innovative
approach has significant potential in the treatment of bone injuries and deformities by providing
tailored solutions that support better patient outcomes and overall quality of life. This review
summarizes the analysis, biocompatibility, mechanical properties, and potential of promoting
osteogenesis of bioinks and biomaterials for 3D printing in bone tissue engineering. So, this
review encourages interdisciplinary collaboration and supports innovation in regenerative
medicine.
2023-01-01T00:00:00Z